Search results for " Proteomic analysis"
showing 10 items of 12 documents
Comparative protEome profiling and functional analysis of chronic myelogenous leucemia cell lines
2008
Myc Promoter-Binding Protein-1 (MBP-1) Is a Novel Potential Prognostic Marker in Invasive Ductal Breast Carcinoma
2010
BackgroundAlpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATA-box binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features ha…
SWATH-MS reveals a key role for IPO7 in the molecular mechanism underlying antitumor effects of curcumin on Chronic Myelogenous Leukemia cells
2016
Imatinib represents the elective drug for the treatment of patients with Chronic Myelogenous Leukemia (CML). However, albeit it is effective in controlling CML and preventing progression to blast crisis (BC), it is not curative. Therefore, it is mandatory to find novel therapeutic combinations to completely eradicate CML. It was described that CML cells expressing higher level of BCR-ABL show an increased glucose metabolism (termed aerobic glycolysis or Warburg effect) correlated with a non-hypoxic induction of HIF-1α, factor implicated in leukemia stem cells (LSCs) maintenance[1,2]. In the present study, we demonstrated that curcumin, an Indian spice with multiple anticancer properties, is…
Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived…
2017
Abstract We have previously isolated exosome-like nanoparticles from Citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. In order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. Among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the Acetyl-CoA Carboxylase 1 (ACACA) and we demonstrated that silencing ACACA in cancer cells leads to a reduction of cell growth. Our study proved that the anti-tumor effects of Citrus-limon nanovesicles is partly mediated by lipid metabolism inhibition, in particular via ACACA downregulation.…
Biological activities of AgNPs-EPS on breast cancer cells in vitro
2016
IMPROVED PRODUCTION OF THE ANTIBIOTIC ACTINORHODIN IN STREPTOMYCES COELICOLOR IMMOBILIZED-MYCELIAL CELL CULTIVATIONS
2017
Objectives i) Evaluation of ACT production in Streptomyces coelicolor M145 mycelial cells immobilized on polycaprolactone (PCL) and polylactic acid (PLA) nanofiber membranes, modified or not by an O2- plasma treatment. ii) Identification of gene products associated with the improvement of ACT production.
A "fat" Streptomycete strain overproduces antibiotics
DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY
2015
The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…
Rôle de la Dynamique Membranaire dans la Mise en Place des Mécanismes de Défense chez le Tabac
2011
Cryptogein, a protein secreted by the oomycete Phytophthora cryptogea, induces defense mechanisms in tobacco. Several proteins involved in the associated signaling pathway were identified and localized on the plasma membrane (PM). A fraction resistant to solubilization by detergent named DIMs for Detergent Insoluble Membranes, enriched in sterols an sphigolipids had been isolated from tobacco PM. It was proved to contain proteins previously identified as actors of the signaling cascade triggered by cryptogein. In animal cells, the dynamic association of proteins to sterol and sphingolipid rich microdomains under the influence of a biological stimulus plays an essential role in the regulatio…